621
621 .
PYGV AGAR
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Geodermatophilus sp. DSM 44400
* Modification: 8x (8 times the amount of peptone, yeast and glucose)
Growth conditions: aerobic
15 °C
pH 7.20
Main sol. 621 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Mineral salt solution
("Hutner/Cohen-Bazire") |
20 | ml | - | - | |
|
Peptone*
(Bacto) |
2 | g | - | - | |
|
Yeast extract*
(Bacto) |
2 | g | - | - | |
|
Agar
(Bacto) |
15 | g | 15 | 44.599 | |
| Distilled water* | 895 | ml | - | - | |
| 1 Sterilize 20 min./121°C. After cooling to 60°C add to the medium: | |||||
|
Glucose*
(2.5%, sterile-filtered) |
80 | ml | - | - | |
| Vitamin solution (double conc.) | 5 | ml | - | - | |
| 2 Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH). | |||||
Solution occurs in 3
other media (first defined in Medium 621).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
10.00 | g | 10.526 | 55.071 | |
| MgSO4 x 7 H2O | 29.70 | g | 31.263 | 126.841 | |
| CaCl2 x 2 H2O | 3.34 | g | 3.516 | 23.915 | |
| Na2MoO4 x 2 H2O | 12.67 | mg | 0.013 | 0.055 | |
| FeSO4 x 7 H2O | 99.00 | mg | 0.104 | 0.375 | |
|
Metall salt sol. 44
(see medium 590) |
50.00 | ml | - | - | |
| Distilled water* | 900.00 | ml | - | - | |
| 1 Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water. | |||||
Solution occurs in 6
other media (first defined in Medium 621).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Biotin | 4.0 | mg | 0.004 | 0.016 | |
| Folic acid | 4.0 | mg | 0.004 | 0.009 | |
| Pyridoxine hydrochloride | 20.0 | mg | 0.02 | 0.097 | |
| Riboflavine | 10.0 | mg | 0.01 | 0.027 | |
| Thiamine-HCl x 2 H2O | 10.0 | mg | 0.01 | 0.027 | |
| Nicotinamide | 10.0 | mg | 0.01 | 0.082 | |
| D-Calcium pantothenate | 10.0 | mg | 0.01 | - | |
| Vitamin B12 | 0.2 | mg | 2.0e-4 | 1.5e-4 | |
| p-Aminobenzoic acid | 10.0 | mg | 0.01 | 0.073 | |
| Distilled water* | 1000.0 | ml | - | - | |
| 1 Store in the dark and cold (5°C). | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| MgSO4 x 7 H2O | 0.625 | 2.537 |
| Glucose | 0.25 | 1.388 |
| Peptone | 0.25 | - |
| Yeast extract | 0.25 | - |
| Show more | ||
