621 .
PYGV AGAR
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Equipment needed:
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Mineral salt solution
("Hutner/Cohen-Bazire") |
20.00 | ml | ||
|
|
Peptone
(Bacto) |
0.25 | g | ||
|
|
Yeast extract
(Bacto) |
0.25 | g | ||
|
|
Agar
(Bacto) |
15.00 | g | ||
|
|
Distilled water | 965.00 | ml | ||
| 1 Sterilize 20 min./121°C. After cooling to 60°C add to the medium: | |||||
|
|
Glucose
(2.5%, sterile-filtered) |
10.00 | ml | ||
|
|
Vitamin solution (double conc.) | 5.00 | ml | ||
| 2 Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH). | |||||
Mineral salt solution #
950
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid
(NTA) |
10.00 | g | ||
|
|
MgSO4 x 7 H2O | 29.70 | g | ||
|
|
CaCl2 x 2 H2O | 3.34 | g | ||
|
|
Na2MoO4 x 2 H2O | 12.67 | mg | ||
|
|
FeSO4 x 7 H2O | 99.00 | mg | ||
|
|
Metall salt sol. 44
(see medium 590) |
50.00 | ml | ||
|
|
Distilled water | 900.00 | ml | ||
| 1 Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water. | |||||
Vitamin solution (double conc.) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Biotin | 4.0 | mg | ||
|
|
Folic acid | 4.0 | mg | ||
|
|
Pyridoxine hydrochloride | 20.0 | mg | ||
|
|
Riboflavine | 10.0 | mg | ||
|
|
Thiamine-HCl x 2 H2O | 10.0 | mg | ||
|
|
Nicotinamide | 10.0 | mg | ||
|
|
D-Calcium pantothenate | 10.0 | mg | ||
|
|
Vitamin B12 | 0.2 | mg | ||
|
|
p-Aminobenzoic acid | 10.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Store in the dark and cold (5°C). | |||||