DSMZ 629 . STALEY'S MAINTENANCE MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5.0 g 5 -
Yeast extract 0.5 g 0.5 -
Hutner's salts 20.0 ml - -
Vitamin solution
(filter-sterilized)
10.0 ml - -
Distilled water 970.0 ml - -
Agar , for solid medium 15.0 g/l 15 44.599
1 Dissolve peptone and yeast extract in the distilled water, adjust the pH to 7.2 and sterilize. After the medium has cooled add the Hutner's salts and Vitamin solution.
2 For solid medium use 15.0 g agar.
Solution occurs in 24 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 -
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
Metall salt sol. 44 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
Solution occurs in 1 other media (first defined in Medium 600).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
Biotin 2.0 mg 0.002 0.008
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Calcium pantothenate 5.0 mg 0.005 0.01
Folic acid 2.0 mg 0.002 0.005
Riboflavin 5.0 mg 0.005 0.013
Nicotinamide 9.0 mg 0.009 0.074
Distilled water 1000.0 ml - -
1 Filter-sterilized.
Solution occurs in 18 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Peptone 5 -
MgSO4 x 7 H2O 0.594 2.41
Yeast extract 0.5 -
Nitrilotriacetic acid 0.2 1.046
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain