630
630 .
MODIFIED THERMUS 162 MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 630 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 2.5 | g | 2.5 | - | |
| Tryptone | 2.5 | g | 2.5 | 35.172 | |
| Agar | 28.0 | g | 28 | 83.252 | |
| Nitrilotriacetic acid | 100.0 | mg | 0.1 | 0.523 | |
| CaSO4 x 2 H2O | 40.0 | mg | 0.04 | 0.232 | |
| MgCl2 x 6 H2O | 200.0 | mg | 0.2 | 0.984 | |
|
Fe(III) citrate
(0.01 M) |
0.5 | ml | 0.001 | 0.005 | |
| Trace element solution | 0.5 | ml | - | - | |
| Phosphate buffer | 100.0 | ml | - | - | |
| Distilled water | 900.0 | ml | - | - | |
| 1 Adjust pH to 7.2 with NaOH. Autoclave at 121°C for 15 min. Autoclave the phosphate buffer separately and then add to the medium. | |||||
Solution occurs in 3
other media (first defined in Medium 630).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 5.44 | g | 5.44 | 39.975 | |
| Na2HPO4 x 12 H2O | 43.00 | g | 43 | 302.904 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.2 | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 12.8 | g | 12.8 | 66.967 | |
| FeCl2 x 4 H2O | 1.0 | g | 1 | 5.03 | |
| MnCl2 x 4 H2O | 0.5 | g | 0.5 | 2.526 | |
| CoCl2 x 4 H2O | 0.3 | g | 0.3 | 2.311 | |
| CuCl2 x 2 H2O | 50.0 | mg | 0.05 | 0.293 | |
| Na2MoO4 x 2 H2O | 50.0 | mg | 0.05 | 0.207 | |
| H3BO3 | 20.0 | mg | 0.02 | 0.323 | |
| NiCl2 x 6 H2O | 20.0 | mg | 0.02 | 0.154 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 8 - 9 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 28 | 83.252 |
| Na2HPO4 x 12 H2O | 4.3 | 30.29 |
| Tryptone | 2.5 | 35.172 |
| Yeast extract | 2.5 | - |
| KH2PO4 | 0.544 | 3.998 |
| Show more | ||
Cultivation metadata from 14 strains
| pH | 6 - 8 | 1 strain |
| phyla | 3 | 14 strains |
| oxygen | aerobe | 1 strain |
| temperature | thermophilic | 14 strains |