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Culture medium recipe

Equipment needed: Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
dehydrated RCM medium
(dissolved in distilled water)
1000.0 ml - -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 -
1 Dissolve dehydrated RCM medium (available from BD-BBL, OXOID or MERCK) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.


Last modified: 11.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 6

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium resazurin 5.0e-4 -
dehydrated RCM medium - -

Cultivation metadata from 6 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 8 strains
oxygen anaerobe 6 strains
temperature mesophilic 6 strains
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