634
634 .
RCM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Clostridium butyricum DSM 10702
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Main sol. 634 #
ml
Equipment needed: Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
dehydrated RCM medium
(dissolved in distilled water) |
1000.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| 1 Dissolve dehydrated RCM medium (available from BD-BBL, OXOID or MERCK) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. | |||||
Metadata
| Last modified: | 11.10.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 7 |
Clostridium butyricum DSM 10702
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sodium resazurin | 5.0e-4 | - |
| dehydrated RCM medium | - | - |
