634 .
RCM MEDIUM
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Selected strain: Clostridium chauvoei DSM 7528
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Equipment needed:
Autoclave
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
6.8
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
dehydrated RCM medium
(dissolved in distilled water) |
1000.0 | ml | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | ||
| 1 Dissolve dehydrated RCM medium (available from BD-BBL, OXOID or MERCK) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. | |||||