Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
dehydrated RCM medium
(dissolved in distilled water) |
1000.0 | ml | ||
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | ||
1 Dissolve dehydrated RCM medium (available from BD-BBL, OXOID or MERCK) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. |