634b
634b .
RCM MEDIUM (with Sea Salts)
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Clostridium aestuarii DSM 19521
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
30 °C
Main sol. 634b #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| dehydrated RCM medium | 38.0 | g | 38 | - | |
|
Sea salts
(SIGMA) |
30.0 | g | 30 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve dehydrated RCM medium (available from BD BBL, OXOID or MERCK) and SIGMA Sea Salts (30.0 g/l) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Dispense the medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Adjust pH of complete medium to 6.8. | |||||
Metadata
| Last modified: | 11.10.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Clostridium aestuarii DSM 19521
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| dehydrated RCM medium | 38 | - |
| Sea salts | 30 | - |
| Sodium resazurin | 5.0e-4 | 0.002 |
| Distilled water | - | - |
