634c
634c .
RCM MEDIUM (N2/CO2)
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 634c #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| dehydrated RCM medium | 38.0 | g | 38 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Sodium carbonate | 1.0 | g | 1 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve dehydrated RCM medium (available from BD BBL, OXOID, or MERCK) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v). Bring to a boil and cool to room temperature while sparging with an 80% N2 and 20% CO2 gas mixture. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave for 15 min at 121°C. Before use, add 1.0 g of sodium carbonate per liter medium from a sterile anoxic stock solution prepared under an 80% N2 and 20% CO2 gas mixture. Check the pH of the medium after autoclaving and adjust the pH to 6.8 if necessary. | |||||
Metadata
| Last modified: | 13.03.25 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| dehydrated RCM medium | 38 | - |
| Sodium carbonate | 1 | - |
| Sodium resazurin | 5.0e-4 | 0.002 |
| Distilled water | - | - |
Cultivation metadata from 18 strains
| pH | 6 - 8 | 3 strains |
| pH | 8 - 10 | 2 strains |
| pH | 4 - 6 | 1 strain |
| phyla | 3 | 18 strains |
| oxygen | anaerobe | 10 strains |
| Show more | ||