DSMZ 634c . RCM MEDIUM (N2/CO2)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
dehydrated RCM medium 38.0 g 38 -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 0.002
Sodium carbonate 1.0 g 1 -
Distilled water 1000.0 ml - -
1 Dissolve dehydrated RCM medium (available from BD BBL, OXOID or MERCK) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use, add 1.0 g of sodium carbonate per liter medium from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Check pH of medium after autoclaving and adjust pH to 6.8, if necessary.

Metadata

Last modified: 11.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 11
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
dehydrated RCM medium 38 -
Sodium carbonate 1 -
Sodium resazurin 5.0e-4 0.002
Distilled water - -

Cultivation metadata from 11 strains BacDive

pH 6 - 8 3 strains
pH 8 - 10 2 strains
pH 4 - 6 1 strain
phyla 2 10 strains
oxygen anaerobe 7 strains
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