63b
63b .
DESULFOVIBRIO MEDIUM (SEAWATER)
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 63b #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution A | 980 | ml | - | - | |
| Solution B | 10 | ml | - | - | |
| Solution C | 10 | ml | - | - | |
| 1 Dissolve ingredients of solution A, bring to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under 100% N2 gas into anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C. Adjust pH of the complete medium to 6.8 - 7.0, if necessary. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.5 | g | 0.51 | 2.929 | |
| NH4Cl | 1.0 | g | 1.02 | 19.077 | |
| Na2SO4 | 1.0 | g | 1.02 | 7.184 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.102 | 0.694 | |
| MgSO4 x 7 H2O | 2.0 | g | 2.041 | 8.28 | |
| Na-DL-lactate | 2.0 | g | 2.041 | 18.212 | |
| Yeast extract | 1.0 | g | 1.02 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
|
Sea water
(filtered and aged) |
980.0 | ml | - | - | |
| 1 Alternatively to filtered aged sea water bottled water from Biomaris GmbH (Bremen, Germany) can be used. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeSO4 x 7 H2O | 0.5 | g | 50 | 179.846 |
| Distilled water | 10.0 | ml | - | - |
Equipment needed: Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na-thioglycolate | 0.1 | g | 10 | 87.643 |
| Ascorbic acid | 0.1 | g | 10 | 56.78 |
| Distilled water | 10.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 - 7.0 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na-DL-lactate | 2 | 17.848 |
| MgSO4 x 7 H2O | 2 | 8.114 |
| NH4Cl | 1 | 18.695 |
| Na2SO4 | 1 | 7.04 |
| Yeast extract | 1 | - |
| Show more | ||
