63c .
DESULFOVIBRIO MEDIUM (CHOLINE)
What is this page?
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
read more
Final pH:
6.8 - 7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 980 | ml | ||
|
|
Solution B | 10 | ml | ||
|
|
Solution C | 10 | ml | ||
| 1 Dissolve ingredients of solution A, bring to the boil, then cool to room temperature while sparging with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under 100% N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C. Check medium pH after autoclaving and adjust to 6.8 - 7.0, if necessary. | |||||
Solution A #
980
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
K2HPO4 | 0.5 | g |
|
|
NH4Cl | 1.0 | g |
|
|
Na2SO4 | 1.0 | g |
|
|
CaCl2 x 2 H2O | 0.1 | g |
|
|
MgSO4 x 7 H2O | 2.0 | g |
|
|
Choline chloride
(SIGMA) |
5.0 | g |
|
|
Yeast extract | 1.0 | g |
|
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml |
|
|
Distilled water | 980.0 | ml |
Solution C #
10
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na-thioglycolate | 0.1 | g |
|
|
Ascorbic acid | 0.1 | g |
|
|
Distilled water | 10.0 | ml |