DSMZ 640a . THERMOCLOSTRIDIUM CAENICOLA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Selected strain: Thermoclostridium caenicola DSM 19027

This strain uses the standard medium without modifications.

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.90 g 0.9 16.826
NaCl 0.90 g 0.9 15.4
MgCl2 x 6 H2O 0.40 g 0.4 1.968
KH2PO4 0.75 g 0.75 5.511
K2HPO4 1.50 g 1.5 8.612
Trypticase peptone
(BD BBL)
2.00 g 2 -
Yeast extract
(OXOID)
1.00 g 1 -
Trace element solution SL-10 1.00 ml - -
FeCl3 x 6 H2O
(0.1% w/v in 0.2 N HCl)
2.50 ml 0.003 0.009
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
NaHCO3 2.00 g 2 23.808
Cellobiose 1.00 g 1 2.921
L-Cysteine HCl x H2O 0.50 g 0.5 2.847
Distilled water 1000.00 ml - -
1 Dissolve ingredients except bicarbonate, cellobiose and cysteine. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then add bicarbonate and adjust pH to 6.8. Distribute medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cellobiose and cysteine after autoclaving from anoxic stock solutions prepared under 100% N2 gas atmosphere. Cellobiose has to be sterilized by filtration. Adjust pH of the complete medium to 6.5, if necessary.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 05.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Thermoclostridium caenicola DSM 19027

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2 23.808
Trypticase peptone 2 -
K2HPO4 1.5 8.612
Cellobiose 1 2.921
Yeast extract 1 -
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain