DSMZ 641 . DESULFOVIBRIO (MV) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 0.988 18.473
Na2SO4 2.0 g 1.976 13.914
Na2S2O3 x 5 H2O 1.0 g 0.988 3.981
MgSO4 x 7 H2O 1.0 g 0.988 4.009
CaCl2 x 2 H2O 0.1 g 0.099 0.672
KH2PO4 0.5 g 0.494 3.631
Trace element solution SL-10 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Yeast extract 1.0 g 0.988 -
Sodium resazurin
(0.1% w/v)
0.5 ml 4.9e-4 0.002
NaHCO3 2.0 g 1.976 23.525
Wolin's vitamin solution 10.0 ml - -
Na-DL-lactate 2.5 g 2.47 22.045
Na2S x 9 H2O 0.1 g 0.099 0.411
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except bicarbonate, vitamins, lactate and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving complete the medium by adding vitamins (sterilized by filtration), lactate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of the complete medium to 7.0 - 7.2, if necessary.
2 Note: Prior to inoculation 10-20 mg/l sodium dithionite (added from a 5% w/v solution freshly prepared under N2 and filter-sterilized) can be added to the medium to stimulate growth at the beginning.
Solution occurs in 262 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 173 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 356 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 05.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 54
Strains with modifications: 17

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-DL-lactate 2.47 22.045
NaHCO3 1.976 23.525
Na2SO4 1.976 13.914
NH4Cl 0.988 18.473
MgSO4 x 7 H2O 0.988 4.009
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Cultivation metadata from 54 strains BacDive

pH 6 - 8 17 strains
pH 8 - 10 9 strains
pH 4 - 6 5 strains
phyla 3 55 strains
nutrition chemolithoautotroph 2 strains
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