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DSMZ

DSMZ 643 . SEDIMENTIBACTER MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Sedimentibacter hydroxybenzoicus DSM 7310

This strain uses the standard medium without modifications.

Growth conditions: anaerobic 35 °C

Main sol. 643 #

ml
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sodium Potassium phosphate buffer
(0.2 M, pH 7.0) 		100.00 ml - 20
NaCl 10.00 g 9.94 170.095
NH4Cl 0.50 g 0.497 9.292
MgCl2 x 7 H2O 0.10 g 0.099 1.044
CaCl2 x 2 H2O 0.03 g 0.03 0.203
Modified Wolin's mineral solution 5.00 ml - -
Yeast extract 10.00 g 9.94 -
Glycine 0.75 g 0.746 9.931
L-Arginine x HCl 2.10 g 2.087 -
L-Lysine HCl 1.80 g 1.789 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.40 g 0.398 2.264
Na2S x 9 H2O 0.40 g 0.398 1.655
Distilled water 900.00 ml - -
1 Dissolve ingredients except vitamins, cysteine and sulfide, sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Check pH of complete medium and adjust to pH 7.0, if necessary.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 09.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Sedimentibacter hydroxybenzoicus DSM 7310

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 9.94 -
L-Arginine x HCl 2.087 -
L-Lysine HCl 1.789 -
Glycine 0.746 9.931
NH4Cl 0.497 9.292
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain