DSMZ 644 . CATENOCOCCUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 15.00 g 15 256.674
K2HPO4 1.00 g 1 5.741
KH2PO4 0.15 g 0.15 1.102
NH4Cl 0.50 g 0.5 9.348
Na-acetate 0.82 g 0.82 9.996
Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
MgSO4 x 7 H2O 1.00 g 1 4.057
CaCl2 x 2 H2O 0.10 g 0.1 0.68
Yeast extract 0.20 g 0.2 -
D-Glucose 1.00 g 1 5.551
Agar , for solid medium 15.00 g 15 44.599
Distilled water 1000.00 ml - -
1 Dissolve sodium chloride, hydrogenphosphates, ammonium chloride and acetate, adjust pH of solution to 7.0 - 7.2 and autoclave. After autoclaving add trace elements, magnesium sulfate, calcium chloride, yeast extract and glucose from sterile stock solutions. Adjust pH of complete medium to 7.0, if necessary.
Solution occurs in 27 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Metadata

Last modified: 05.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
Agar 15 44.599
K2HPO4 1 5.741
D-Glucose 1 5.551
MgSO4 x 7 H2O 1 4.057
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen facultative anaerobe 1 strain
temperature mesophilic 1 strain