644
644 .
CATENOCOCCUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 644 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 15.00 | g | 15 | 256.674 | |
| K2HPO4 | 1.00 | g | 1 | 5.741 | |
| KH2PO4 | 0.15 | g | 0.15 | 1.102 | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| Na-acetate | 0.82 | g | 0.82 | 9.996 | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | - | - | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| Yeast extract | 0.20 | g | 0.2 | - | |
| D-Glucose | 1.00 | g | 1 | 5.551 | |
| Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve sodium chloride, hydrogenphosphates, ammonium chloride and acetate, adjust pH of solution to 7.0 - 7.2 and autoclave. After autoclaving add trace elements, magnesium sulfate, calcium chloride, yeast extract and glucose from sterile stock solutions. Adjust pH of complete medium to 7.0, if necessary. | |||||
Solution occurs in 28
other media (first defined in Medium 1369).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 | |
| FeSO4 x 7 H2O | 2.20 | g | 2.2 | 7.913 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
| H3BO3 | 0.03 | g | 0.03 | 0.485 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 2 H2O | 0.03 | g | 0.03 | 0.176 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 pH 3.0-4.0 | |||||
Metadata
| Last modified: | 05.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 15 | 256.674 |
| Agar | 15 | 44.599 |
| K2HPO4 | 1 | 5.741 |
| D-Glucose | 1 | 5.551 |
| MgSO4 x 7 H2O | 1 | 4.057 |
| Show more | ||
