Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 15.00 | g | 15 | 256.674 | |
K2HPO4 | 1.00 | g | 1 | 5.741 | |
KH2PO4 | 0.15 | g | 0.15 | 1.102 | |
NH4Cl | 0.50 | g | 0.5 | 9.348 | |
Na-acetate | 0.82 | g | 0.82 | 9.996 | |
Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | - | - | |
MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
Yeast extract | 0.20 | g | 0.2 | - | |
D-Glucose | 1.00 | g | 1 | 5.551 | |
Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve sodium chloride, hydrogenphosphates, ammonium chloride and acetate, adjust pH of solution to 7.0 - 7.2 and autoclave. After autoclaving add trace elements, magnesium sulfate, calcium chloride, yeast extract and glucose from sterile stock solutions. Adjust pH of complete medium to 7.0, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
EDTA | 5.00 | g | 5 | 17.109 | |
FeSO4 x 7 H2O | 2.20 | g | 2.2 | 7.913 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
H3BO3 | 0.03 | g | 0.03 | 0.485 | |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
CuCl2 x 2 H2O | 0.03 | g | 0.03 | 0.176 | |
NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
Distilled water | 1000.00 | ml | - | - | |
1 pH 3.0-4.0 |
Last modified: | 05.04.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 15 | 256.674 |
Agar | 15 | 44.599 |
K2HPO4 | 1 | 5.741 |
D-Glucose | 1 | 5.551 |
MgSO4 x 7 H2O | 1 | 4.057 |
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