DSMZ 645 . MIDDLEBROOK MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: Mycobacterium decipiens DSM 105360

This strain uses the standard medium without modifications.

Solution occurs in 1 other media (first defined in Medium 645).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Bacto Middlebrook 7H10 agar
(Difco 262710)
19 g 20.995 -
Glycerol 5 ml - -
Distilled water 900 ml - -
1 Sterilize for 15 min by 121°C cool to 50 - 55°C. Add 100 ml Middlebrook OADC enrichment (Becton Dickinson 212240) under aseptic conditions and mix thoroughly.
2 If you can´t order the commercial media then prepare the media in this way:
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.500 g 0.5 3.784
KH2PO4 1.500 g 1.5 11.023
Na2HPO4 1.500 g 1.5 10.566
Sodium citrate 0.400 g 0.4 1.55
MgSO4 0.025 g 0.025 0.208
CaCl2 0.500 mg 5.0e-4 0.005
ZnSO4 1.000 mg 1.0e-3 0.006
CuSO4 1.000 mg 1.0e-3 0.006
L-Glutamic acid 0.500 g 0.5 3.398
Ferric ammonium citrate 0.040 g 0.04 -
Pyridoxine 1.000 mg 1.0e-3 0.006
Biotin 0.500 mg 5.0e-4 0.002
Malachite green 0.250 mg 2.5e-4 6.9e-4
Agar
(Bacto )
15.000 g 15 44.599
Distilled water 1000.000 ml - -
1 Final pH at 25°C 6.6 ± 0.2.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Oleic acid 0.05 g 0.5 1.77
Bovine albumin
(albumin fraction V)
5.00 g 50 -
Dextrose 2.00 g 20 100.923
Catalase
(beef)
4.00 mg 0.04 -
NaCl 0.85 g 8.5 145.448
Distilled water 100.00 ml - -

Metadata

Last modified: 20.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 323
Mycobacterium decipiens DSM 105360

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Bacto Middlebrook 7H10 agar 20.995 -
Glycerol - -
Distilled water - -

Cultivation metadata from 323 strains BacDive

pH 6 - 8 9 strains
pH 4 - 6 3 strains
phyla 2 319 strains
oxygen aerobe 63 strains
oxygen microaerophile 29 strains
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