Loading ...

645. MIDDLEBROOK MEDIUM

Culture medium recipe

Vol.
×
Solution occurs in 1 other media (first defined in medium 645 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Bacto Middlebrook 7H10 agar
(Difco 262710)
19 g - -
2 5 ml 6.269 68.068
3 900 ml 893.917 49619.9
4 Bacto Middlebrook OADC enrichment 100 ml - -
Instructions:
  1. Sterilize for 15 min by 121°C cool to 50 - 55°C. Add 100 ml Middlebrook OADC enrichment (Becton Dickinson 212240) under aseptic conditions and mix thoroughly.
  2. If you can´t order the commercial media then prepare the media in this way:
Solution occurs in 1 other media (first defined in medium 645 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.500 g 0.5 3.784
2 1.500 g 1.5 11.023
3 1.500 g 1.5 10.566
4 0.400 g 0.4 1.55
5 0.025 g 0.025 0.208
6 0.500 mg 5.0e-4 0.005
7 1.000 mg 1.0e-3 0.006
8 1.000 mg 1.0e-3 0.006
9 0.500 g 0.5 3.398
10 0.040 g 0.04 -
11 1.000 mg 1.0e-3 0.006
12 0.500 mg 5.0e-4 0.002
13 0.250 mg 2.5e-4 6.9e-4
14 15.000 g 15 -
15 1000.000 ml 998.207 55408.8
Instructions:
  1. Final pH at 25°C 6.6 ± 0.2.
Solution occurs in 1 other media (first defined in medium 645 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.05 g 0.5 1.77
2 5.00 g 50 -
3 2.00 g 20 111.015
4
(beef)
4.00 mg 0.04 -
5 0.85 g 8.5 145.442
6 100.00 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 308
Strains with modifications: 7
Mycolicibacterium stellerae DSM 45590

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 1012.11 56180.7
Glycerol 6.269 68.068
Albumin fraction V, bovine 4.975 -
Dextrose 1.99 11.046
NaCl 0.846 14.472
Show more

Cultivation metadata from 308 strains BacDive

halophily moderate 20 strains
halophily not 5 strains
halophily slightly 4 strains
pH 6 - 8 9 strains
pH 4 - 6 4 strains
Show more

Similar media

780. MIDDLEBROOK MEDIUM WITH MYCOBACTIN 95 %
911. MIDDLEBROCK 7H11 AGAR 72 %
857. BACTO MIDDLEBROOK 7H9 BROTH 72 %

Latest comments

No comments for this medium.