648
648 .
CLOSTRIDIUM GRANTII MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 648 #
ml
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Marine Broth
(BD Difco) |
18.5 | g | 18.5 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| Na2CO3 | 1.0 | g | 1 | 9.435 | |
| D-Glucose | 5.0 | g | 5 | 27.753 | |
| L-Cysteine HCl x H2O | 0.3 | g | 0.3 | 1.708 | |
| Na2S x 9 H2O | 0.3 | g | 0.3 | 1.249 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve DIFCO Marine Broth powder and resazurin, bring the medium to a boil, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Dispense the medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Complete the medium by adding glucose, cysteine, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The pH of the complete medium should be around 6.6. | |||||
Metadata
| Last modified: | 25.05.23 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.6 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Marine Broth | 18.5 | - |
| D-Glucose | 5 | 27.753 |
| Na2CO3 | 1 | 9.435 |
| L-Cysteine HCl x H2O | 0.3 | 1.708 |
| Na2S x 9 H2O | 0.3 | 1.249 |
| Show more | ||
