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DSMZ

DSMZ 651 . R 8 A H MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 651 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Malic acid 2.50 g 2.5 18.644
Yeast extract
(Difco 0127) 		1.00 g 1 -
(NH4)2SO4 1.25 g 1.25 9.46
MgSO4 x 7 H2O 0.20 g 0.2 0.811
CaCl2 x 2 H2O 0.07 g 0.07 0.476
Fe(III) citrate 0.01 g 0.01 0.041
Na2-EDTA 0.02 g 0.02 0.059
KH2PO4 0.60 g 0.6 4.409
K2HPO4 0.90 g 0.9 5.167
Trace elements 1.00 ml - -
Vitamin solution 7.50 ml - -
Distilled water 1000.00 ml - -
1 Neutralize malic acid with NaOH and adjust the pH of the completed medium for a final pH to 6.9.

Trace elements #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(III) citrate 0.30 g 3 12.248
MnSO4 x H2O 2.00 mg 0.02 0.118
H3BO3 1.00 mg 0.01 0.162
CuSO4 x 5 H2O 1.00 mg 0.01 0.04
(NH4)6Mo7O24 x 4 H2O 2.00 mg 0.02 -
ZnSO4 1.00 mg 0.01 0.062
EDTA 0.05 g 0.5 1.711
CaCl2 x 2 H2O 0.02 g 0.2 1.36
Distilled water 100.00 ml - -

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nicotinic acid 0.2 g 0.2 1.625
Nicotinamide 0.2 g 0.2 1.638
Thiamine-HCl x 2 H2O 0.4 g 0.4 1.072
Biotin 8.0 mg 0.008 0.033
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.9
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Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Malic acid 2.5 18.644
(NH4)2SO4 1.25 9.46
Yeast extract 1 -
K2HPO4 0.9 5.167
KH2PO4 0.6 4.409
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
oxygen obligate aerobe 1 strain
temperature mesophilic 1 strain