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DSMZ

DSMZ 660 . ALCALIGENES MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Cupriavidus metallidurans DSM 8374

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 660 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Tris 6.06 g 6.06 50.025
NaCl 4.68 g 4.68 80.082
KCl 1.49 g 1.49 19.987
NH4Cl 1.07 g 1.07 20.004
Na2SO4 0.43 g 0.43 3.027
MgCl2 x 6 H2O 0.20 g 0.2 0.984
CaCl2 x 2 H2O 0.03 g 0.03 0.204
Na2HPO4 x 12 H2O 0.23 g 0.23 1.62
Fe(NH4)citrate 5.00 mg 0.005 -
Trace element solution SL-7 1.00 ml - -
Distilled water 988.50 ml - -
1 After autoclaving add 2.5 ml 1.0 M CuSO4 and 10 ml of a 40% Na-succinate solution, both sterilized by filtration.
CuSO4
(1 M, sterilized by filtration) 		2.50 ml 0.399 2.5
Sodium succinate
(40 %, sterilized by filtration) 		10.00 ml 4 24.684

Trace element solution SL-7 #

1000
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		1 ml 0.25 6.857
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 60 mg 0.06 0.97
CoCl2 x 6 H2O 200 mg 0.2 1.54
CuCl2 x 2 H2O 20 mg 0.02 0.117
NiCl2 x 6 H2O 20 mg 0.02 0.154
Na2MoO4 x 2 H2O 40 mg 0.04 0.165
Distilled water 1000 ml - -

Metadata

Last modified: 23.11.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Filter
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Associated strains

All strains for this medium: 2
Cupriavidus metallidurans DSM 8374

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tris 6.06 50.025
NaCl 4.68 80.082
Sodium succinate 4 24.684
KCl 1.49 19.987
NH4Cl 1.07 20.004
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains