DSMZ 663 . DESULFITOBACTERIUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 5.44 g 5.44 39.975
MgCl2 x 2 H2O 0.18 g 0.18 1.372
CaCl2 x 2 H2O 0.03 g 0.03 0.204
NH4Cl 0.50 g 0.5 9.348
Trace element solution 5.00 ml - -
Yeast extract 1.00 g 1 -
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 -
Na-pyruvate 2.50 g 2.5 22.718
Na2S2O3 x 5 H2O 2.00 g 2 8.058
Wolin's vitamin solution 20.00 ml - -
L-Cysteine HCl x H2O 0.40 g 0.4 2.277
Na2S x 9 H2O 0.40 g 0.4 1.665
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except pyruvate, thiosulfate, vitamins, cysteine and sulfide), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add pyruvate, thiosulfate, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of pyruvate and vitamins are sterilized by filtration. Adjust medium pH to 7.5 by adding sterile anoxic 1 N NaOH.
Solution occurs in 8 other media (first defined in Medium 144).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
12.80 g 12.8 66.967
FeCl2 x 4 H2O 0.20 g 0.2 1.006
MnCl2 x 4 H2O 0.10 g 0.1 0.505
CoCl2 x 6 H2O 0.17 g 0.17 1.309
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnCl2 0.10 g 0.1 0.734
CuCl2 0.02 g 0.02 0.149
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
NaCl 1.00 g 1 17.112
Na2SeO3 x 5 H2O 0.03 g 0.03 0.114
Distilled water 1000.00 ml - -
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 05.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 5.44 39.975
Na-pyruvate 2.5 22.718
Na2S2O3 x 5 H2O 2 8.058
Yeast extract 1 -
NH4Cl 0.5 9.348
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain