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665. SULFOBACILLUS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 700 ml - -
2 Solution B 301 ml - -
3 Solution C 20 ml - -
Instructions:
  1. After autoclaving, combine the three solutions. Medium pH 1.9 - 2.4.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.00 g 4.286 32.433
2 0.10 g 0.143 1.916
3 0.50 g 0.714 4.101
4 0.50 g 0.714 2.898
5 0.01 g 0.014 0.087
6 700.00 ml 998.207 55408.8
Instructions:
  1. Adjust pH to 2.0 - 2.2 with sulfuric acid.
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 44.2 g 146.844 528.187
2 300.0 ml 994.891 55224.8
3
(10 N)
1.0 ml - -
Instructions:
  1. sterilize by filtration or handle under N2 before autoclaving to prevent oxidation of the iron.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(1% w/v in water)
20 ml 10 -

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 2.1
Equipment needed: Filter

Associated strains

All strains for this medium: 5

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 977.676 54269.2
FeSO4 x 7 H2O 43.291 155.714
(NH4)2SO4 2.938 22.236
K2HPO4 0.49 2.812
MgSO4 x 7 H2O 0.49 1.987
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Cultivation metadata from 5 strains BacDive

pH 0 - 2 1 strain
pH 2 - 4 1 strain
phyla 1 5 strains
oxygen aerobe 1 strain
temperature thermophilic 5 strains

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