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DSMZ

DSMZ 665 . SULFOBACILLUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 665 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 700 ml - -
Solution B 301 ml - -
Solution C 20 ml - -
1 After autoclaving, combine the three solutions. Medium pH 1.9 - 2.4.

Solution A #

700
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 3.00 g 4.286 32.433
KCl 0.10 g 0.143 1.916
K2HPO4 0.50 g 0.714 4.101
MgSO4 x 7 H2O 0.50 g 0.714 2.898
Ca(NO3)2 0.01 g 0.014 0.087
Distilled water 700.00 ml - -
1 Adjust pH to 2.0 - 2.2 with sulfuric acid.

Solution B #

301
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 44.2 g 146.844 528.187
Distilled water 300.0 ml - -
H2SO4 , 10 N 1.0 ml - -
1 (sterilize by filtration or handle under N2 before autoclaving to prevent oxidation of the iron)

Solution C #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(1% w/v in water) 		20 ml 0.2 -

Metadata

Last modified: 13.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 1.9 - 2.4
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 6

Molecular composition

Compound Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 43.291 155.714
(NH4)2SO4 2.938 22.236
K2HPO4 0.49 2.812
MgSO4 x 7 H2O 0.49 1.987
KCl 0.098 1.314
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Cultivation metadata from 6 strains BacDive

pH 0 - 2 1 strain
pH 2 - 4 1 strain
phyla 1 5 strains
oxygen aerobe 1 strain
temperature thermophilic 5 strains