Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Solution A | 700 | ml | ||
|
Solution B | 301 | ml | ||
|
Solution C | 20 | ml | ||
1 After autoclaving, combine the three solutions. Medium pH 1.9 - 2.4. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
(NH4)2SO4 | 3.00 | g | ||
|
KCl | 0.10 | g | ||
|
K2HPO4 | 0.50 | g | ||
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
Ca(NO3)2 | 0.01 | g | ||
|
Distilled water | 700.00 | ml | ||
1 Adjust pH to 2.0 - 2.2 with sulfuric acid. |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
FeSO4 x 7 H2O | 44.2 | g | ||
|
Distilled water | 300.0 | ml | ||
|
H2SO4 , 10 N | 1.0 | ml | ||
1 (sterilize by filtration or handle under N2 before autoclaving to prevent oxidation of the iron) |