670
670 .
ACIDIFERROBACTER (MS) MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Acidiphilium cryptum DSM 9467
* Modification: Adjust pH to 3.0 with 1 N H2SO4 prior to autoclaving and omit FeSO4 x 7 H2O from the medium. Before use, add 2.00 g/l D-glucose and 0.1 g/l yeast extract from filter-sterilized stock solutions.
Growth conditions: 37 °C
Main sol. 670 #
ml
Equipment needed: Autoclave
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 2.00 | g | 2 | 15.136 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.014 | |
| K2HPO4 | 0.10 | g | 0.1 | 0.574 | |
| KCl | 0.10 | g | 0.1 | 1.341 | |
|
FeSO4 x 7 H2O*
(40% w/v in 0.2 N H2SO4) |
50.00 | ml | 20 | 71.939 | |
| Yeast extract* | 0.10 | g | - | - | |
| D-Glucose* | 2.00 | g | - | - | |
| Distilled water | 950.00 | ml | - | - | |
| 1 Dissolve ingredients except ferrous sulfate and adjust pH to 2.2 with 4 N H2SO4. Sterilize medium by autoclaving for 15 min at 121°C. Before use, add FeSO4 x 7 H2O from a 40% (w/v) stock solution prepared with 0.2 N H2SO4 and filter-sterilized under 100% N2 gas atmosphere. | |||||
| 2 Note: Incubate statically without shaking. | |||||
Metadata
| Last modified: | 28.11.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | * 3.0 |
| Equipment needed: | Autoclave, Filter, Gassing station |
| Edit in Medium builder | |
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| (NH4)2SO4 | 2 | 15.136 |
| D-Glucose | 2 | 11.101 |
| MgSO4 x 7 H2O | 0.25 | 1.014 |
| KCl | 0.1 | 1.341 |
| K2HPO4 | 0.1 | 0.574 |
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