DSMZ 674 . MEDIUM FOR ACIDURIC, THERMOPHILIC BACILLUS STRAINS

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Selected strain: Bacillus sp. DSM 9197

This strain uses the standard medium without modifications.

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 500 ml - -
Solution B 500 ml - -
1 Solutions A and B are sterilized separately. After cooling to approximately 45°C solution A is poured into solution B. Mix thoroughly.
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.20 g 0.4 3.027
MgSO4 x 7 H2O 0.50 g 1 4.057
CaCl2 x 2 H2O 0.25 g 0.5 3.401
KH2PO4 3.00 g 6 44.09
Yeast extract 1.00 g 2 -
Tryptone
(Bacto)
1.00 g 2 28.137
Glucose 1.00 g 2 11.101
Starch 1.00 g 2 -
Distilled water 500.00 ml - -
1 Adjust pH to 4.3.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Agar 20 g 40 118.931
Distilled water 500 ml - -

Metadata

Last modified: 21.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3
Bacillus sp. DSM 9197

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20 59.466
KH2PO4 3 22.045
Tryptone 1 14.069
Glucose 1 5.551
Yeast extract 1 -
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
temperature thermophilic 3 strains