674
674 .
MEDIUM FOR ACIDURIC, THERMOPHILIC BACILLUS STRAINS
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Bacillus sp. DSM 9197
This strain uses the standard medium without modifications.
Growth conditions: 55 °C
Main sol. 674 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution A | 500 | ml | - | - | |
| Solution B | 500 | ml | - | - | |
| 1 Solutions A and B are sterilized separately. After cooling to approximately 45°C solution A is poured into solution B. Mix thoroughly. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 0.20 | g | 0.4 | 3.027 | |
| MgSO4 x 7 H2O | 0.50 | g | 1 | 4.057 | |
| CaCl2 x 2 H2O | 0.25 | g | 0.5 | 3.401 | |
| KH2PO4 | 3.00 | g | 6 | 44.09 | |
| Yeast extract | 1.00 | g | 2 | - | |
|
Tryptone
(Bacto) |
1.00 | g | 2 | 28.137 | |
| Glucose | 1.00 | g | 2 | 11.101 | |
| Starch | 1.00 | g | 2 | - | |
| Distilled water | 500.00 | ml | - | - | |
| 1 Adjust pH to 4.3. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Agar | 20 | g | 40 | 118.931 |
| Distilled water | 500 | ml | - | - |
Metadata
| Last modified: | 21.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Bacillus sp. DSM 9197
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 20 | 59.466 |
| KH2PO4 | 3 | 22.045 |
| Tryptone | 1 | 14.069 |
| Glucose | 1 | 5.551 |
| Yeast extract | 1 | - |
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