DSMZ 68 . HALOTHIOBACILLUS NEAPOLITANUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 4.0 g 4 29.393
K2HPO4 4.0 g 4 22.965
MgSO4 x 7 H2O 0.8 g 0.8 3.246
NH4Cl 0.4 g 0.4 7.478
Trace element solution (Vishniac and Santer, 1957) 5.0 ml - -
Bromocresol purple
(saturated aqueous solution)
0.5 ml - -
Agar , for solid medium
(Bacto)
optional
15.0 g 15 44.599
Na2S2O3 x 5 H2O 10.0 g 10 40.292
Distilled water 1000.0 ml - -
1 Dissolve ingredients except thiosulfate, dispense into suitable cultivation vessels and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution. Adjust pH of complete medium to 6.6 - 7.0.
Solution occurs in 4 other media (first defined in Medium 69).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 22.00 g 22 76.506
CaCl2 x 2 H2O 5.54 g 5.54 37.683
MnCl2 x 4 H2O 5.06 g 5.06 25.568
FeSO4 x 7 H2O 5.00 g 5 17.985
(NH4)6Mo7O24 x 4 H2O 1.10 g 1.1 0.89
CuSO4 x 5 H2O 1.57 g 1.57 6.288
CoCl2 x 6 H2O 1.61 g 1.61 12.4
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.

Metadata

Last modified: 31.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.6 - 7.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar
optional
15 44.599
Na2S2O3 x 5 H2O 10 40.292
KH2PO4 4 29.393
K2HPO4 4 22.965
MgSO4 x 7 H2O 0.8 3.246
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Cultivation metadata from 3 strains BacDive

pH 2 - 4 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 3 strains
nutrition chemoautolithotroph 1 strain
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