Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 4.0 | g | 4 | 29.393 | |
K2HPO4 | 4.0 | g | 4 | 22.965 | |
MgSO4 x 7 H2O | 0.8 | g | 0.8 | 3.246 | |
NH4Cl | 0.4 | g | 0.4 | 7.478 | |
Trace element solution (Vishniac & Santer, 1957) | 5.0 | ml | - | - | |
Bromocresol purple
(saturated aqueous solution) |
0.5 | ml | - | - | |
Agar
, for solid medium
(Bacto) optional |
15.0 | g | 15 | 44.599 | |
Na2S2O3 x 5 H2O | 10.0 | g | 10 | 40.292 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve ingredients except thiosulfate, dispense into suitable cultivation vessels and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution. Adjust pH of complete medium to 6.6 - 7.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 50.00 | g | 50 | 148.718 | |
ZnSO4 x 7 H2O | 22.00 | g | 22 | 76.506 | |
CaCl2 x 2 H2O | 5.54 | g | 5.54 | 37.683 | |
MnCl2 x 4 H2O | 5.06 | g | 5.06 | 25.568 | |
FeSO4 x 7 H2O | 5.00 | g | 5 | 17.985 | |
(NH4)6Mo7O24 x 4 H2O | 1.10 | g | 1.1 | 0.89 | |
CuSO4 x 5 H2O | 1.57 | g | 1.57 | 6.288 | |
CoCl2 x 6 H2O | 1.61 | g | 1.61 | 12.4 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH. |
Last modified: | 31.03.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 6.6 - 7.0 |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 3 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar optional |
15 | 44.599 |
Na2S2O3 x 5 H2O | 10 | 40.292 |
KH2PO4 | 4 | 29.393 |
K2HPO4 | 4 | 22.965 |
MgSO4 x 7 H2O | 0.8 | 3.246 |
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