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69. STARKEYA NOVELLA MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2 Solution A 900 ml - -
3 Solution B 50 ml - -
4 Solution C 5 ml - -
5 Solution D 50 ml - -
6 , for pH adjustment
(sterile 0.5 N)
x - -
Instructions:
  1. Solutions A, B, C and D are sterilized separately by autoclaving and mixed when cooled to room temperature. Adjust pH of complete medium to 8.5 with sterile 0.5 N NaOH.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10.6 g 11.752 32.813
2 1.5 g 1.663 12.22
3 0.3 g 0.333 6.218
4 0.3 g 0.333 -
5
(0.1% w/v)
2.0 ml 0.002 0.006
6
(if required)
15.0 g 16.63 -
7 900.0 ml 995.994 55286
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.1 g 2 8.114
2 50.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution (Vishniac and Santer, 1957) 5 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 g 100 402.924
2 50 ml 998.207 55408.8
Solution occurs in 4 other media (first defined in medium 69 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 50.00 g 50 148.718
2 22.00 g 22 76.506
3 5.54 g 5.54 37.683
4 5.06 g 5.06 25.568
5 5.00 g 5 17.985
6 1.10 g 1.1 0.89
7 1.57 g 1.57 6.288
8 1.61 g 1.61 6.767
9 1000.00 ml 998.207 55408.8
10 , for pH adjustment
(2 N)
x - -
Instructions:
  1. Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.5

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 996.225 55298.8
Bacto Agar 14.892 -
Na2HPO4 x 12 H2O 10.524 29.385
Na2S2O3 x 5 H2O 4.975 20.046
KH2PO4 1.489 10.943
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains

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