DSMZ 69 . STARKEYA NOVELLA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 900 ml - -
Solution B 50 ml - -
Solution C 5 ml - -
Solution D 50 ml - -
1 Solutions A, B, C and D are sterilized separately by autoclaving and mixed when cooled to room temperature. Adjust pH of complete medium to 8.5 with sterile 0.5 N NaOH.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 10.6 g 11.752 82.782
KH2PO4 1.5 g 1.663 12.22
NH4Cl 0.3 g 0.333 6.218
Yeast extract 0.3 g 0.333 -
Phenol red
(0.1% w/v)
2.0 ml 0.002 0.006
Agar , for solid medium
(Bacto)
optional
15.0 g 16.63 49.445
Distilled water 900.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.1 g 2 8.114
Distilled water 50.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution (Vishniac and Santer, 1957) 5 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 5 g 100 402.924
Distilled water 50 ml - -
Solution occurs in 4 other media (first defined in Medium 69).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 22.00 g 22 76.506
CaCl2 x 2 H2O 5.54 g 5.54 37.683
MnCl2 x 4 H2O 5.06 g 5.06 25.568
FeSO4 x 7 H2O 5.00 g 5 17.985
(NH4)6Mo7O24 x 4 H2O 1.10 g 1.1 0.89
CuSO4 x 5 H2O 1.57 g 1.57 6.288
CoCl2 x 6 H2O 1.61 g 1.61 12.4
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar
optional
14.967 44.5
Na2HPO4 x 12 H2O 10.577 74.504
Na2S2O3 x 5 H2O 5 20.146
KH2PO4 1.497 10.998
NH4Cl 0.299 5.596
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains