DSMZ 691 . CreDm1 MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.40 g 1.4 10.288
NH4Cl 0.50 g 0.5 9.348
MgCl2 x 6 H2O 0.20 g 0.2 0.984
CaCl2 x 2 H2O 0.15 g 0.15 1.02
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Wolin's vitamin solution 10.00 ml - -
NaHCO3 1.50 g 1.5 17.856
Na2-DL-malate 1.60 g 1.6 8.986
Distilled water 1000.00 ml - -
1 Dissolve ingredients except vitamins, bicarbonate and malate. Adjust pH to 6.5, dispense medium in culture vessels and autoclave. Prior to inoculation add vitamins, bicarbonate and malate from stock solutions sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.9.
Solution occurs in 262 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 173 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 356 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 06.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.7 - 6.9
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2-DL-malate 1.6 8.986
NaHCO3 1.5 17.856
KH2PO4 1.4 10.288
NH4Cl 0.5 9.348
MgCl2 x 6 H2O 0.2 0.984
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen facultative anaerobe 1 strain
temperature psychrophilic 1 strain