DSMZ 691 . CreDm1 MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Selected strain: unclassified bacterium DSM 6526

This strain uses the standard medium without modifications.

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.40 g 1.396 10.257
NH4Cl 0.50 g 0.499 9.32
MgCl2 x 6 H2O 0.20 g 0.199 0.981
CaCl2 x 2 H2O 0.15 g 0.15 1.017
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
NaHCO3 1.50 g 1.496 17.802
Na2-DL-malate 1.60 g 1.595 8.959
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients except vitamins, bicarbonate and malate. Adjust pH to 6.5, dispense medium in culture vessels and autoclave. Prior to inoculation add vitamins, bicarbonate and malate from stock solutions sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.9.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 176 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 299 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 06.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.7 - 6.9
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1
unclassified bacterium DSM 6526

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2-DL-malate 1.595 8.959
NaHCO3 1.496 17.802
KH2PO4 1.396 10.257
NH4Cl 0.499 9.32
MgCl2 x 6 H2O 0.199 0.981
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen facultative anaerobe 1 strain
temperature psychrophilic 1 strain