691 .
CreDm1 MEDIUM
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Selected strain: DSM 6526
This strain uses the standard medium without modifications.
Growth conditions: facultative anaerobic
20 °C
Equipment needed:
Autoclave
Filter
Final pH:
6.7 - 6.9
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 1.40 | g | ||
|
|
NH4Cl | 0.50 | g | ||
|
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MgCl2 x 6 H2O | 0.20 | g | ||
|
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CaCl2 x 2 H2O | 0.15 | g | ||
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Trace element solution SL-10 | 1.00 | ml | ||
|
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Selenite-tungstate solution | 1.00 | ml | ||
|
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NaHCO3 | 1.50 | g | ||
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Na2-DL-malate | 1.60 | g | ||
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Wolin's vitamin solution (10x) | 1.00 | ml | ||
|
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Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients except vitamins, bicarbonate and malate. Adjust pH to 6.5, dispense medium in culture vessels and autoclave. Prior to inoculation add vitamins, bicarbonate and malate from stock solutions sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.9. | |||||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
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ZnCl2 | 70.0 | mg | ||
|
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MnCl2 x 4 H2O | 100.0 | mg | ||
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H3BO3 | 6.0 | mg | ||
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CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
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NiCl2 x 6 H2O | 24.0 | mg | ||
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Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
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Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.5 | g |
|
|
Na2SeO3 x 5 H2O | 3.0 | mg |
|
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Na2WO4 x 2 H2O | 4.0 | mg |
|
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Distilled water | 1000.0 | ml |
Wolin's vitamin solution (10x) #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 20 | mg |
|
|
Folic acid | 20 | mg |
|
|
Pyridoxine hydrochloride | 100 | mg |
|
|
Thiamine HCl | 50 | mg |
|
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Riboflavin | 50 | mg |
|
|
Nicotinic acid | 50 | mg |
|
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Calcium D-(+)-pantothenate | 50 | mg |
|
|
Vitamin B12 | 1 | mg |
|
|
p-Aminobenzoic acid | 50 | mg |
|
|
(DL)-alpha-Lipoic acid | 50 | mg |
|
|
Distilled water | 1000 | ml |