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692. METHANOSALSUM WEN5 MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 15.0 g 14.735 252.124
2 10.0 g 9.823 116.933
3 0.3 g 0.295 2.166
4 0.5 g 0.491 9.182
5 Trace element solution 10.0 ml - -
6
(0.1% w/v)
2.0 ml 0.002 0.008
7 0.5 g 0.491 -
8
(0.1% w/v)
0.5 ml 4.9e-4 0.002
9 4.0 g 3.929 37.073
10 5.0 ml 3.88 121.096
11 1.0 g 0.982 4.09
12 1000.0 ml 980.557 54429.1
Instructions:
  1. Dissolve ingredients except carbonate, methanol and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate and adjust pH to 9.0 - 9.2, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add methanol and sulfide from anoxic stock solutions autoclaved under 100% N2 gas. Adjust pH of complete medium to 9.2 - 9.4.
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
17 , required for pH adjustment x - -
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 9.4
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Light

1 strain needs light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 990.363 54973.4
NaCl 14.745 252.292
NaHCO3 9.823 116.933
Na2CO3 3.929 37.073
Methanol 3.88 121.096
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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