DSMZ 693 . COLUMBIA BLOOD MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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* Modified for Actinobacillus succinogenes DSM 22257

For reactivation distribute the complete content of the ampoule on one agar plate

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Columbia agar base 1000 ml - -
Defibrinated sheep blood 5 % 50 -
1 Columbia agar base supplemented with 5% defibrinated sheep blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below).
2 ATTENTION: organisms, for which only M 693 is mentioned in the catalogue entry, do not grow in one of the media listed below! Those are meant to rehydrate the freeze-dried pellets, not for cultivation.
Solution occurs in 4 other media (first defined in Medium 54a).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 17.0 g 17 -
Soy peptone 3.0 g 3 -
D(+)-Glucose 2.5 g 2.5 13.877
NaCl 5.0 g 5 85.558
K2HPO4 2.5 g 2.5 14.353
Distilled water 1000.0 ml - -
1 Adjust pH to 7.3.
2 The same as Caso Bouillon (Merck or Oxoid, Germany).
Solution occurs in 2 other media (first defined in Medium 429).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Brain heart infusion
(Difco)
37 g 37 -
Distilled water 1000 ml - -
Solution occurs in 2 other media (first defined in Medium 429).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Distilled water 1000 ml - -
1 Adjust pH to 7.0.

Metadata

Last modified: 24.11.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 1981
Strains with modifications: 87
Actinobacillus succinogenes DSM 22257

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Defibrinated sheep blood 50 -
Columbia agar base - -

Cultivation metadata from 1981 strains BacDive

halophily halotolerant 1 strain
halophily non-halophilic 1 strain
pH 6 - 8 60 strains
pH 8 - 10 31 strains
pH 4 - 6 28 strains
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