DSMZ 693 . COLUMBIA BLOOD MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Selected strain: Salmonella enterica subsp. enterica DSM 107298

This strain uses the standard medium without modifications.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Columbia agar base 1000 ml - -
Defibrinated sheep blood 5 % 50 -
1 Columbia agar base supplemented with 5% defibrinated sheep blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below).
2 ATTENTION: organisms, for which only M 693 is mentioned in the catalogue entry, do not grow in one of the media listed below! Those are meant to rehydrate the freeze-dried pellets, not for cultivation.
Solution occurs in 4 other media (first defined in Medium 54a).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 17.0 g 17 -
Soy peptone 3.0 g 3 -
D(+)-Glucose 2.5 g 2.5 13.877
NaCl 5.0 g 5 85.558
K2HPO4 2.5 g 2.5 14.353
Distilled water 1000.0 ml - -
1 Adjust pH to 7.3.
2 The same as Caso Bouillon (Merck or Oxoid, Germany).
Solution occurs in 2 other media (first defined in Medium 429).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Brain heart infusion
(Difco)
37 g 37 -
Distilled water 1000 ml - -
Solution occurs in 2 other media (first defined in Medium 429).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Distilled water 1000 ml - -
1 Adjust pH to 7.0.

Metadata

Last modified: 24.11.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 2013
Strains with modifications: 87
Salmonella enterica subsp. enterica DSM 107298

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Defibrinated sheep blood 50 -
Columbia agar base - -

Cultivation metadata from 2013 strains BacDive

halophily halotolerant 1 strain
halophily non-halophilic 1 strain
pH 6 - 8 60 strains
pH 8 - 10 31 strains
pH 4 - 6 28 strains
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