699 .
TRIPLE-SUGAR-IRON MEDIUM (Difco)
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Equipment needed:
Final pH:
7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Bacto beef extract | 3.000 | g | ||
|
|
Yeast extract
(Bacto ) |
3.000 | g | ||
|
|
Bacto peptone | 15.000 | g | ||
|
|
Dextrose | 1.000 | g | ||
|
|
Lactose | 10.000 | g | ||
|
|
Sucrose | 10.000 | g | ||
|
|
FeSO4 | 0.200 | g | ||
|
|
NaCl | 5.000 | g | ||
|
|
Na2S2O3 | 0.300 | g | ||
|
|
Phenol red | 0.024 | g | ||
|
|
Agar , if required | 12.000 | g | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 pH 7.4 | |||||