DSMZ 701 . HALOANAEROBACTER CHITINOVORANS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 1 18.695
MgCl2 x 6 H2O 7.0 g 7 34.431
MgSO4 x 7 H2O 9.6 g 9.6 38.949
CaCl2 x 2 H2O 0.5 g 0.5 3.401
KCl 3.8 g 3.8 50.973
K2HPO4 x 3 H2O 0.4 g 0.4 2.297
NaCl 10.0 g 10 171.116
NaHCO3 3.0 g 3 35.711
Na2CO3 1.0 g 1 9.435
Na2S x 9 H2O 0.5 g 0.5 2.082
Yeast extract 1.0 g 1 -
L-Cysteine 0.5 g 0.5 4.127
Resazurin 1.0 mg 1.0e-3 0.004
Substrate
(glucose, chitin etc.)
5.0 g 5 -
Modified Wolin's mineral solution II 1.0 ml - -
Distilled water 1000.0 ml - -
1 pH 7.2
2 Prepare the medium without the Na2S, Na2CO3, NaHCO3, L-cysteine and substrate. Boil the medium and cool under a stream of nitrogen. Add the Na2CO3 and NaHCO3 . Adjust the pH to 7.2. If chitin is used as the substrate it must be added before autoclaving. Autoclave the medium and add the Na2S (from a sterile, neutralized stock solution prepared under nitrogen), L-cysteine (from a stock solution prepared under nitrogen), and the glucose or N-acetylglucosamine (if used as substrate) from sterile stock solution prepared under nitrogen. Check the final pH.
Solution occurs in 22 other media (first defined in Medium 700).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g - -
MgSO4 x 7 H2O 3.000 g - -
MnSO4 x H2O 0.500 g - -
NaCl 1.000 g - -
FeSO4 x 7 H2O 0.100 g - -
CoSO4 x 7 H2O 0.180 g - -
CaCl2 x 2 H2O 0.100 g - -
ZnSO4 x 7 H2O 0.180 g - -
CuSO4 x 5 H2O 0.010 g - -
AlK(SO4)2 x 12 H2O 0.020 g - -
H3BO3 0.010 g - -
Na2MoO4 x 2 H2O 0.010 g - -
NiCl2 x 6 H2O 0.025 g - -
Na2SeO3 x 5 H2O 0.300 mg - -
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 7 34.431
Substrate 5 -
KCl 3.8 50.973
NaHCO3 3 35.711
NH4Cl 1 18.695
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain