Name: HALOANAEROBACTER CHITINOVORANS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 701 #

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NH₄Cl	1.0	g	1	18.695
MgCl₂ x 6 H₂O	7.0	g	7	34.431
MgSO₄ x 7 H₂O	9.6	g	9.6	38.949
CaCl₂ x 2 H₂O	0.5	g	0.5	3.401
KCl	3.8	g	3.8	50.973
K₂HPO₄ x 3 H₂O	0.4	g	0.4	2.297
NaCl	10.0	g	10	171.116
NaHCO₃	3.0	g	3	35.711
Na₂CO₃	1.0	g	1	9.435
Na₂S x 9 H₂O	0.5	g	0.5	2.082
Yeast extract	1.0	g	1	-
L-Cysteine	0.5	g	0.5	4.127
Resazurin	1.0	mg	1.0e-3	0.004
Substrate (glucose, chitin etc.)	5.0	g	5	-
Modified Wolin's mineral solution II	1.0	ml	-	-
Distilled water	1000.0	ml	-	-
1 pH 7.2
2 Prepare the medium without the Na₂S, Na₂CO₃, NaHCO₃, L-cysteine and substrate. Boil the medium and cool under a stream of nitrogen. Add the Na₂CO₃ and NaHCO₃ . Adjust the pH to 7.2. If chitin is used as the substrate it must be added before autoclaving. Autoclave the medium and add the Na₂S (from a sterile, neutralized stock solution prepared under nitrogen), L-cysteine (from a stock solution prepared under nitrogen), and the glucose or N-acetylglucosamine (if used as substrate) from sterile stock solution prepared under nitrogen. Check the final pH.

Modified Wolin's mineral solution II #

1000

Solution occurs in 24 other media (first defined in Medium 700).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Nitrilotriacetic acid	1.500	g	1.5	7.848
MgSO₄ x 7 H₂O	3.000	g	3	12.172
MnSO₄ x H₂O	0.500	g	0.5	2.958
NaCl	1.000	g	1	17.112
FeSO₄ x 7 H₂O	0.100	g	0.1	0.36
CoSO₄ x 7 H₂O	0.180	g	0.18	1.161
CaCl₂ x 2 H₂O	0.100	g	0.1	0.68
ZnSO₄ x 7 H₂O	0.180	g	0.18	0.626
CuSO₄ x 5 H₂O	0.010	g	0.01	0.04
AlK(SO₄)₂ x 12 H₂O	0.020	g	0.02	0.042
H₃BO₃	0.010	g	0.01	0.162
Na₂MoO₄ x 2 H₂O	0.010	g	0.01	0.041
NiCl₂ x 6 H₂O	0.025	g	0.025	0.193
Na₂SeO₃ x 5 H₂O	0.300	mg	3.0e-4	0.001
Distilled water	1000.000	ml	-	-
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

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