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DSMZ

DSMZ 704 . BUTYRIVIBRIO SP. MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 704 #

ml
Equipment needed: Centrifuge Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral solution 75.00 ml - -
Clarified rumen fluid 150.00 ml - -
K2HPO4 0.30 g 0.3 1.721
Trypticase peptone
(BD BBL) 		2.00 g 1.998 -
Yeast extract
(OXOID) 		2.00 g 1.998 -
Volatile fatty acid mixture 3.10 ml - -
Haemin solution
(0.05% w/v) 		2.00 ml - -
Glycerol 0.50 g 0.5 5.424
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 4.00 g 3.996 37.702
D-Glucose 1.00 g 0.999 5.545
Maltose 1.00 g 0.999 2.919
Cellobiose 1.00 g 0.999 2.919
Starch
(soluble) 		1.00 g 0.999 -
L-Cysteine HCl x H2O 0.25 g 0.25 1.422
Na2S x 9 H2O 0.25 g 0.25 1.04
Distilled water 770.00 ml - -
1 Dissolve ingredients (except carbonate, carbohydrates, cysteine and sulfide) and sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Add the carbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add glucose, maltose, cellobiose, starch, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Cellobiose should be sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.8, if necessary.

Mineral solution #

1000
Solution occurs in 4 other media (first defined in Medium 330).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 6.0 g 6 44.09
NaCl 12.0 g 12 205.339
(NH4)2SO4 6.0 g 6 45.406
CaCl2 x 2 H2O 1.6 g 1.6 10.883
MgSO4 x 7 H2O 2.5 g 2.5 10.143
Distilled water 1000.0 ml - -

Clarified rumen fluid #

1000
Solution occurs in 28 other media (first defined in Medium 1310).
1 Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.

Volatile fatty acid mixture #

1000
Solution occurs in 4 other media (first defined in Medium 330).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Acetic acid 548.50 ml 573.182 9545.09
Propionic acid 193.50 ml 192.145 2593.76
Butyric acid 129.00 ml - -
n-Valeric acid 32.25 ml 30.283 296.512
iso-Butyric acid 32.25 ml 30.638 347.719
DL-2-Methylbutyric acid 32.25 ml - -
iso-Valeric acid 32.25 ml 30.025 293.986

Haemin solution #

100
Solution occurs in 15 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Haemin 50 mg 0.5 0.767
NaOH
(1 N) 		1 ml - -
Distilled water 100 ml - -
1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

Metadata

Last modified: 31.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.7 - 6.8
Equipment needed: Autoclave, Centrifuge, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 12

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 3.996 37.702
Yeast extract 1.998 -
Trypticase peptone 1.998 -
D-Glucose 0.999 5.545
Cellobiose 0.999 2.919
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Cultivation metadata from 12 strains BacDive

phyla 1 11 strains
oxygen anaerobe 10 strains
temperature mesophilic 11 strains