Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
(NH4)2SO4 | 0.40 | g | 0.4 | 3.027 | |
K2HPO4 | 0.20 | g | 0.2 | 1.148 | |
KCl | 0.10 | g | 0.1 | 1.341 | |
FeSO4 x 7 H2O | 0.01 | g | 0.01 | 0.036 | |
Yeast extract | 0.25 | g | 0.25 | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except yeast extract and adjust pH to 2.0 with H2SO4. After autoclaving add yeast extract from a sterile stock solution. | |||||
2 Note: For autotrophic growth omit yeast extract and add to the medium 13.90 g/l FeSO4 x 7 H2O. Adjust medium pH to 1.7 with H2SO4 prior to autoclaving. |
Last modified: | 06.04.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 2.0 |
Equipment needed: | Autoclave |
Edit in Medium builder |
All strains for this medium: | 2 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
MgSO4 x 7 H2O | 0.5 | 2.029 |
(NH4)2SO4 | 0.4 | 3.027 |
Yeast extract | 0.25 | - |
K2HPO4 | 0.2 | 1.148 |
KCl | 0.1 | 1.341 |
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