709
709 .
ACIDIMICROBIUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Acidimicrobium ferrooxidans DSM 10331
This strain uses the standard medium without modifications.
Growth conditions: 45 °C
Main sol. 709 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
| (NH4)2SO4 | 0.40 | g | 0.4 | 3.027 | |
| K2HPO4 | 0.20 | g | 0.2 | 1.148 | |
| KCl | 0.10 | g | 0.1 | 1.341 | |
| FeSO4 x 7 H2O | 0.01 | g | 0.01 | 0.036 | |
| Yeast extract | 0.25 | g | 0.25 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except yeast extract and adjust pH to 2.0 with H2SO4. After autoclaving add yeast extract from a sterile stock solution. | |||||
| 2 Note: For autotrophic growth omit yeast extract and add to the medium 13.90 g/l FeSO4 x 7 H2O. Adjust medium pH to 1.7 with H2SO4 prior to autoclaving. | |||||
Metadata
| Last modified: | 06.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 2.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2 |
Acidimicrobium ferrooxidans DSM 10331
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| MgSO4 x 7 H2O | 0.5 | 2.029 |
| (NH4)2SO4 | 0.4 | 3.027 |
| Yeast extract | 0.25 | - |
| K2HPO4 | 0.2 | 1.148 |
| KCl | 0.1 | 1.341 |
| Show more | ||
