709 .
ACIDIMICROBIUM MEDIUM
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Equipment needed:
Autoclave
Final pH:
2.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
|
(NH4)2SO4 | 0.40 | g | ||
|
|
K2HPO4 | 0.20 | g | ||
|
|
KCl | 0.10 | g | ||
|
|
FeSO4 x 7 H2O | 0.01 | g | ||
|
|
Yeast extract | 0.25 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients except yeast extract and adjust pH to 2.0 with H2SO4. After autoclaving add yeast extract from a sterile stock solution. | |||||
| 2 Note: For autotrophic growth omit yeast extract and add to the medium 13.90 g/l FeSO4 x 7 H2O. Adjust medium pH to 1.7 with H2SO4 prior to autoclaving. | |||||