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71. ACIDITHIOBACILLUS THIOOXIDANS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.00 g 3 22.045
2 0.50 g 0.5 2.029
3 3.00 g 3 22.703
4 0.25 g 0.25 1.701
5 5.00 g 5 20.146
6 1000.00 ml 998.207 55408.8
7
optional
5.00 g/l 5 16.532
8 , might need strain adjustment 10.00 mg/l - -
9 , might need strain adjustment 10.00 g/l - -
Instructions:
  1. Prepare the medium without thiosulfate, adjust pH to 4.4 - 4.7 and autoclave at 121°C for 15 min. Sterilize thiosulfate separately by filtration and add after autoclaving.
  2. Note: Incubate statically without shaking.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 4.5
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 6
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Na2S2O3 x 5 H2O 5 20.146
K2S4O6
optional
5 16.532
(NH4)2SO4 3 22.703
KH2PO4 3 22.045
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Cultivation metadata from 6 strains BacDive

phyla 1 6 strains
temperature mesophilic 6 strains

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