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710. THERMOANAEROBACTER (KOKO) MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(BD Bacto)
1.00 g 0.988 -
2
(meat)
1.00 g 0.988 -
3
(BD Bacto)
1.00 g 0.988 -
4 1.60 g 1.581 9.077
5 1.00 g 0.988 6.334
6 0.50 g 0.494 9.236
7 0.16 g 0.158 0.692
8 Trace element solution SL-11 1.00 ml - -
9
(0.1% w/v)
0.50 ml 4.9e-4 0.002
10 0.06 g 0.059 0.403
11 1.00 g 0.988 11.763
12 5.00 g 4.941 27.425
13 Vitamin solution 10.00 ml - -
14 0.30 g 0.296 1.688
15 0.30 g 0.296 1.234
16 1000.00 ml 986.371 54751.8
Instructions:
  1. Dissolve ingredients (except calcium chloride, bicarbonate, glucose, vitamins, cysteine and sulfide), adjust pH to 7.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add calcium chloride, glucose, cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas atmosphere. Add bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are prepared under 100% N2 gas and sterilized by filtration. The pH of the complete medium should be at 7.0.
  2. Note: Supplementation of medium with 10.00 g/l MOPS buffer (pH 6.9 - 7.0; 10%(w/v) anoxic stock solution) may enhance the buffer capacity of the medium.
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.221 55354.1
D-Glucose 4.941 27.425
K2HPO4 1.581 9.077
NaHCO3 0.988 11.763
NaH2PO4 x 2 H2O 0.988 6.334
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature thermophilic 2 strains

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