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711a. EUBACTERIUM AGGREGANS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 mod. medium 711 (medium 711a) 1000 ml - -
Instructions:
  1. Use medium 711 with 1g/l yeast extract, without acetate, and with 5 g/l fructose as the substrate.
Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 1 18.695
2 0.30 g 0.3 1.722
3 0.30 g 0.3 2.205
4 0.20 g 0.2 0.984
5 0.10 g 0.1 0.68
6 0.10 g 0.1 1.341
7 0.60 g 0.6 10.267
8 1.00 g 1 -
9
(0.1% w/v)
0.50 ml 5.0e-4 0.002
10 1.50 g 1.5 14.153
11 Trace element solution SL-10 1.50 ml - -
12 Vitamin solution 10.00 ml - -
13
(TMC)
1.19 g 1.19 4.995
14 0.30 g 0.3 1.708
15 0.30 g 0.3 1.249
16 1000.00 ml 998.207 55408.8
18 5.00 g/l 5 -
Instructions:
  1. Dissolve ingredients (except carbonate, vitamins, TMC and reducing agents) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add vitamins, TMC, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The vitamin solution should be sterilized by filtration. The stock solution of TMC (6% w/v) should be neutralized with NaOH prior to sterilization by filtration. Adjust pH of complete medium to 7.0, if necessary.
  2. Look for further instructions at medium 711.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 1009.67 56045.2
Fructose 5 -
Na2CO3 1.5 14.153
trans-3,4,5-trimethoxycinnamate 1.19 4.995
NH4Cl 1 18.695
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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