MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 716 . AMMONIFEX DEGENSII MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 716 #

ml
Equipment needed: Autoclave Gassing station Filter Serum bottle
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 x 3 H2O 0.30 g 0.299 1.717
KH2PO4 0.22 g 0.219 1.612
(NH4)2SO4 0.22 g 0.219 1.66
NaCl 1.00 g 0.997 17.06
MgSO4 x 7 H2O 0.09 g 0.09 0.364
CaCl2 x 2 H2O 0.06 g 0.06 0.407
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) 		2.00 ml 0.002 0.007
NiCl2 x 6 H2O
(0.1% w/v) 		0.20 ml 2.0e-4 0.002
Modified Wolin's mineral solution 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
KNO3 1.00 g 0.997 9.861
Na2CO3 1.50 g 1.496 14.11
DL-Dithiothreitol 0.30 g 0.299 1.938
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except nitrate, carbonate, and dithiothreitol), then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic serum vials (e.g., 10 ml medium in 25 ml Balch-type tubes) and autoclave. Before use, add nitrate from a sterile anoxic stock solution prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Before inoculation add dithiothreitol from an anoxic stock solution sterilized by filtration under 100% N2 gas and adjust the pH of the medium to 7.2.
2 After growth has started pressurize vials to 0.5 bar overpressure with a sterile 80% H2 and 20% CO2 gas mixture.

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Last modified: 20.06.23
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Gassing station, Serum bottle
Edit in Medium builder

Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 1.496 14.11
KNO3 0.997 9.861
DL-Dithiothreitol 0.299 1.938
K2HPO4 x 3 H2O 0.299 1.717
(NH4)2SO4 0.219 1.66
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain