71 .
ACIDITHIOBACILLUS THIOOXIDANS MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
4.4 - 4.7
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 3.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
|
(NH4)2SO4 | 3.00 | g | ||
|
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
|
Na2S2O3 x 5 H2O | 5.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Prepare the medium without thiosulfate, adjust pH to 4.4 - 4.7 and autoclave at 121°C for 15 min. Sterilize thiosulfate separately by filtration and add after autoclaving. | |||||
| 2 Note: Incubate statically without shaking. | |||||