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DSMZ

DSMZ 720 . DESULFITOBACTERIUM HAFNIENSE MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Desulfitobacterium hafniense DSM 12420

This strain uses the standard medium without modifications.

Growth conditions: anaerobic 37 °C

Main sol. 720 #

ml
Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.00 g 0.997 18.639
NaCl 0.10 g 0.1 1.706
MgCl2 x 6 H2O 0.10 g 0.1 0.49
CaCl2 x 2 H2O 0.05 g 0.05 0.339
K2HPO4 x 3 H2O 0.40 g 0.399 2.29
Trace element solution SL-11 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Yeast extract 1.00 g 0.997 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
NaHCO3 2.60 g 2.592 30.857
Na-pyruvate 2.50 g 2.493 22.65
Na2S2O3 x 5 H2O 1.25 g 1.246 5.021
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.30 g 0.299 1.245
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, pyruvate, thiosulfate, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add bicarbonate and adjust pH to 7.0 - 7.2, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to use add pyruvate, thiosulfate, vitamins, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of pyruvate and vitamins are sterilized by filtration.

Trace element solution SL-11 #

1000
Solution occurs in 22 other media (first defined in Medium 722).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 5.2 g 5.2 15.467
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 06.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4
Desulfitobacterium hafniense DSM 12420

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.592 30.857
Na-pyruvate 2.493 22.65
Na2S2O3 x 5 H2O 1.246 5.021
NH4Cl 0.997 18.639
Yeast extract 0.997 -
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Cultivation metadata from 4 strains BacDive

pH 6 - 8 1 strain
phyla 1 4 strains
oxygen anaerobe 4 strains
oxygen obligate anaerobe 1 strain
temperature mesophilic 4 strains