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722. DESULFOVIBRIO GABONENSIS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.0 g 2.994 21.078
2 0.3 g 0.299 4.016
3 0.3 g 0.299 5.597
4 0.2 g 0.2 1.467
5 0.1 g 0.1 0.679
6 50.0 g 49.9 853.834
7 3.3 g 3.293 16.2
8 1.6 g 1.597 6.479
9 0.1 g 0.1 -
10 Trace element solution SL-11 1.0 ml - -
11
(0.1% w/v)
0.5 ml 5.0e-4 0.002
12 1.5 g 1.497 14.124
13 2.5 g 2.495 22.265
14 Seven vitamins solution 1.0 ml - -
15 0.2 g 0.2 0.831
16 1000.0 ml 996.215 55298.2
Instructions:
  1. Dissolve ingredients except carbonate, lactate, vitamins and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add lactate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the complete medium should be 7.0 - 7.2.
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 49.9 853.834
MgCl2 x 6 H2O 3.293 16.2
Na2SO4 2.994 21.078
Na lactate 2.495 22.265
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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