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727. CLOSTRIDIUM ULTUNENSE MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.40 g 0.399 2.933
2 0.43 g 0.429 3.023
3 0.30 g 0.299 5.597
4 0.30 g 0.299 5.123
5 0.10 g 0.1 0.679
6 0.10 g 0.1 0.491
7 10.00 g 9.98 -
8 10.00 g 9.98 -
9
(Oxoid)
5.00 g 4.99 -
10
(soluble)
0.40 g 0.399 -
11 0.50 mg 5.0e-4 0.002
12 0.40 g 0.399 4.866
13 Trace element solution 1.00 ml - -
14 Selenite-tungstate solution 1.00 ml - -
15 Vitamin solution 10.00 ml - -
16
(10% w/v solution)
50.00 ml 4.99 59.4
17 0.30 g 0.299 1.247
18 940.00 ml 936.442 51980.4
Instructions:
  1. Adjust final medium pH to 7.0.
  2. Prepare medium anaerobically under 80% N2 + 20% CO2. Prepare separate anaerobic stock solutions of trace elements (N2), selenite/ tungstate (N2), vitamins (N2), bicarbonate (N2 + CO2), sulfide (N2) and add to the autoclaved medium.
Solution occurs in 4 other media (first defined in medium 732 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 1.487
2 2.0 g 2 10.06
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 10.0 mg 0.01 0.075
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 1000.0 ml 998.207 55408.8
Instructions:
  1. First dissolve EDTA in distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add remaining compounds.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave

Associated strains

All strains for this medium: No strains found

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 948.397 52644
Peptone 9.98 -
Yeast extract 9.98 -
NaHCO3 4.99 59.4
Lab Lemco powder 4.99 -
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