DSMZ 727 . CLOSTRIDIUM ULTUNENSE MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Gassing station Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.40 g 0.4 2.939
Na2HPO4 0.43 g 0.43 3.029
NH4Cl 0.30 g 0.3 5.609
NaCl 0.30 g 0.3 5.133
CaCl2 x 2 H2O 0.10 g 0.1 0.68
MgCl2 x 6 H2O 0.10 g 0.1 0.492
Yeast extract 10.00 g 10 -
Peptone 10.00 g 10 -
Lab Lemco powder
(Oxoid)
5.00 g 5 -
Starch
(soluble)
0.40 g 0.4 -
Resazurin 0.50 mg 5.0e-4 0.002
Na-acetate 0.40 g 0.4 4.876
Trace element solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Wolin's vitamin solution 10.00 ml - -
NaHCO3
(10% w/v solution, 10 % w/v)
50.00 ml 5 59.519
Na2S x 9 H2O 0.30 g 0.3 1.249
Distilled water 940.00 ml - -
1 Adjust final medium pH to 7.0.
2 Prepare medium anaerobically under 80% N2 + 20% CO2. Prepare separate anaerobic stock solutions of trace elements (N2), selenite/tungstate (N2), vitamins (N2), bicarbonate (N2 + CO2), sulfide (N2) and add to the autoclaved medium.
Solution occurs in 4 other media (first defined in Medium 732).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.5 g 0.5 1.487
FeCl2 x 4 H2O 2.0 g 2 10.06
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
AlCl3 10.0 mg 0.01 0.075
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 First dissolve EDTA in distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add remaining compounds.
Solution occurs in 174 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Peptone 10 -
NaHCO3 5 59.519
Lab Lemco powder 5 -
Na2HPO4 0.43 3.029
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Cultivation metadata from 0 strains BacDive

phyla 1 1 strain