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730. FERROGLOBUS PLACIDUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 940 ml - -
2 Solution B 30 ml - -
3 Solution C 10 ml - -
4 Solution D 10 ml - -
5 Solution E 10 ml - -
Instructions:
  1. Sparge solution A with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic serum vials to 10% volume (e.g., 10 ml medium in 100 ml bottle) and autoclave under 1 bar overpressure of 80% H2 and 20% CO2 gas mixture. Solution B is autoclaved separately after sparging with 80% N2 and 20% CO2 gas mixture. Solution C and D are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solution E is autoclaved under 100% N2 gas atmosphere. To complete the medium add appropriate amounts of solutions B to E to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.0 - 7.2, if necessary. After inoculation pressurize the bottles with 80% H2 and 20% CO2 gas mixture to 2 bar overpressure.
  2. Note: Cultures are very sensitive to the nitrite produced during growth. Thus, incubate this strain ony a few hours at one time (8-12 h) and thereafter check growth by microscopy. Immediately stop the incubation at 85°C, when the medium begins to show some turbidity.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 18.00 g 19.149 327.654
2 4.30 g 4.574 22.501
3 0.14 g 0.149 1.013
4 0.34 g 0.362 4.852
5 0.14 g 0.149 0.855
6 0.24 g 0.255 4.773
7 1.00 g 1.064 10.522
8 Trace element solution 10.00 ml - -
9 930.00 ml 987.588 54819.4
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 50 471.752
2 30.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 10 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1 g 100 908.727
2 10 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.3 g 30 124.905
2 10.0 ml 998.207 55408.8
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 7.1
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
NaCl 18.01 308.166
MgCl2 x 6 H2O 4.3 21.151
Na2CO3 1.5 14.153
KNO3 1 9.891
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain

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